Micropropagation of Rosmarinus Officinalis and Nigella Sativa for Internal Disease Applications

Abstract

This article explores the micropropagation techniques of two highly valued medicinal plants: Rosmarinus officinalis (rosemary) and Nigella sativa (black seed). The study outlines protocols for in vitro cultivation using optimized plant tissue culture methods to produce disease-free and genetically stable plantlets. By utilizing Murashige and Skoog (MS) media with specific growth regulators, efficient shoot proliferation and root induction were achieved. Furthermore, the paper investigates the therapeutic applications of the bioactive compounds derived from these plants—such as rosmarinic acid, carnosic acid, and thymoquinone—in the prevention and treatment of various internal diseases, including inflammatory, oxidative stress-related, and microbial conditions. The integration of micropropagation with hydroponic systems is also discussed as a strategy to enhance the yield and quality of phytochemicals. Overall, the study supports the potential of these plants as natural alternatives or complementary agents in modern medicine and pharmaceutical industries.