Determining The Bioefficacy of An Ointment Containing Levantine Viper Venom

Abstract

The bioefficacy of an anti-inflammatory ointment prepared based on levantine viper venom specifically - the release of the bioactive substance from the base was studied. The membrane diffusion method, an in vitro technique, was employed for this purpose. This method is particularly significant for studying the absorption of a medicinal substance released from an ointment through the skin, due to its convenience and relatively high accuracy. A membrane was used as a skin model. The amount of released substance was determined using HPLC.